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CGP EDU Academic Team
Published on: August 21, 2026
During heat shock to the bacterium, the temperature used for giving thermal shock is
Text Solution
Verified by ExpertsThe correct answer is:
D
In the heat shock method used for transformation of bacteria (like E. coli), cells are briefly exposed to 42°C after being kept on ice. This sudden temperature change helps plasmid DNA enter the bacterial cell.
52°C → too high for transformation
100°C → kills cells
Liquid nitrogen → used in other techniques, not heat shock.
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